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KMID : 0361919910210010097
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Korean Journal of Orthodontics
1991 Volume.21 No. 1 p.97 ~ p.111
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THE EFFECT OF SODIUM FLUORIDE AND SODIUM ORTHOVANADATE ON OSTEOBLASTIC CELL LINE MC3T3-E1 CELLS
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Abstract
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It is the aim of this study to investigate the effects of sodium fluoride and sodium orthovanadate upon the proliferation and activity of the osteoblast (MC3T3-E 1 cells). MC3T3-E 1 cells were cultured in a-MEM containing 10% FBS and various concentration of sodium fluoride and sodium orthovanadate was appended to serum free media. DNA synthesis was examined through the [3H1 thymidine incorporation into DNA. Collagen synthesis was examined through the [3?H] proline incorporation into collagenase digestible protein and noncollagen protein.
The following results were drawn;
1. Sodium fluoride stimulated the DNA synthesis of osteoblast significantly in dose-dependent manner within the concentration from 2puM to 10μM (P < 0.005).
2. Sodium orthovanadate stimulated the DNA synthesis of osteoblast significantly in dosedependent manner within the concentration from 2μM to 8μM, however showed diminution at I0pM (P < 0.001).
3. Sodium fluoride and sodium orthovanadate stimulated the percent collagen synthesis of osteoblast significantly in dose-dependent manner within the concentration from 5μM to 10μM (P < 0.001).
4. Sodium fluoride and sodium orthovanadate stimulated the noncollagen synthesis of osteoblast significnatly in dose-dependent manner within the concentration from 5μM to 10μM (P < 0.001).
In conclusion, sodium fluoride and sodium orthovanadate stimulate the proliferation and activity of osteoblast by stimulation of DNA synthesis and collagen and noncollagen synthesis in osteoblast.
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